L-Ascorbic Acid Sulfate Esters with Special Reference to Enzymatic Hydrolysis

Abstract

1. L-Ascorbic acid 6-sulfate was synthesized as an analog for natural L-ascorbic acid 2-sulfate by the direct sulfation of L-ascorbic acid with pyridine-sulfur trioxide. 2. Its ultraviolet, infrared, and nuclear magnetic resonance spectra were investigated and compared with those of L-ascorbic acid 2-sulfate. 3. A crude sulfatase preparation from the liver of a marine gastropod, Charonia lampas, hydrolyzed L-ascorbic acid 2-sulfate to give L-ascorbate and sulfate. L-Ascorbic acid 6-sulfate was also, although more slowly, hydrolyzed by this enzyme preparation.