IPGMAKER: A program for IBM‐compatible personal computers to create and test recipes for immobilized pH gradients
- 1 January 1990
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 11 (2), 140-147
- https://doi.org/10.1002/elps.1150110207
Abstract
The program “IPGMAKER” is a computational aid for creating and testing recipes for near-linear immobilized pH gradients. It was written for fast IBM personal computers (with a Type 80386 processor and 80387 coprocessor) and compatibles equipped with a VGA, EGA or Hercules (mono) graphics card. The program is limited to the use of up to 10 acids and/or bases, and to ranges spanning between pH 2 and 12. The resulting recipes are presented either as final concentrations in the 2 chambers of a mixing device for linear gradients or as volumes from 0.2 moles/L stock solutions adjusted to a user-defined average buffering power. One of the subroutines determines the pH, gradient slope and buffering capacity at any location of the gradient and includes a facility to estimate the pI of proteins from the composition of their primary structure.This publication has 19 references indexed in Scilit:
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