THE EFFECT OF RIBONUCLEASE DIGESTS OF AMINOACYL-sRNA ON A PROTEIN SYNTHESIS SYSTEM
- 1 November 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (5), 1271-1276
- https://doi.org/10.1073/pnas.52.5.1271
Abstract
Preparations of aminoacyl-sRNA were digested with T1-ribonuclease to produce oligonucleotides with attached aminoacyl groups. Such digests were found to bring about the release of growing polypeptide chains when added to cell-free systems in which polypeptide synthesis was taking place, directed by poly UC. This releasing effect is analogous to the action of puromycin, since evidence was found that amino acids were transferred from the aminoacyl -oligonuleotides to the released chains.Keywords
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