Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis
Open Access
- 1 July 1993
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (14), 4383-4390
- https://doi.org/10.1128/jb.175.14.4383-4390.1993
Abstract
The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologous to that of the prototroph NCDO2118, which was determined previously. Two nonsense mutations and two small deletions were found in the auxotroph sequence, which might explain the branched-chain amino acid auxotrophy. Nevertheless, the leu genes from the auxotroph appear to be transcribed and regulated similarly to those from the prototroph.Keywords
This publication has 33 references indexed in Scilit:
- Nutritional Requirements in Multiple Auxotrophic Lactic Acid Bacteria: Genetic Lesions Affecting Amino Acid Biosynthetic Pathways inLactococcus lactis, Enterococcus faecium, andPediococcus acidilacticiBioscience, Biotechnology, and Biochemistry, 1992
- Genetics of the proteolytic system of lactic acid bacteriaFEMS Microbiology Letters, 1990
- Structure and Expression of the Lactococcus lactis Gene for Phospho- -galactosidase (lacG) in Escherichia coli and L. lactisMicrobiology, 1989
- Proteolytic enzymes of dairy starter culturesFEMS Microbiology Letters, 1987
- Amino acid limited growth of starter cultures in milkFEMS Microbiology Letters, 1987
- Lactose Hydrolysing Enzymes inStreptococcus lactisandStreptococcus cremorisand also in some other Species of StreptococciJournal of Applied Bacteriology, 1980
- Studies of Some Naturally Occurring Auxotrophs of Neisseria gonorrhoeaeMicrobiology, 1980
- Biosynthesis of the parasporal inclusion of Bacillus thuringiensis: half-life of its corresponding messenger RNABiochimie, 1972
- Study of Microbial Evolution through Loss of Biosynthetic Functions: Establishment of “Defective” MutantsNature, 1967
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958