Distribution of class IIDQ antigens on normal and leukemic lymphoid cells

Abstract

The distribution of DQ as well as DR antigens was examined on lymphoid and monocytic cells at different stages of differentiation. In the B cell series, both immature and differentiating (under Staphylococcus aureus or pokeweed mitogen stimulation) B cells often lacked DQ molecules; among surface IgM⁺ and IgD⁺ cells, both DQ and DR molecules were detected except on cells from 30% of the lymphoid malignancies studied. T cells expressed DQ molecules only after stimulation; a DQ⁻DR⁺ phenotype was observed in a large number of cells after allogeneic stimulation, in certain antigen-specific T cell lines as well as in T cell lymphomas, suggesting that class II antigens had a distinct pattern of regulation. In the monocytic lineage, DQ molecules were expressed by most lymph node monocytes and only a low percentage (20%) of circulating monocytes.