CHARACTERIZATION OF TRANSFORMED-CELLS AND TUMORS BY PROTON NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY

Abstract

Cultured acute lymphoblastic leukemic cells [human] give a well-resolved proton NMR spectrum characteristic of isolated plasma membranes. It was demonstrated that the signals, in the spectrum of whole cells, arise predominantly from the plasma membrane and cells transformed by pokeweed mitogen have membranes which are significantly less rigid than are normal human peripheral blood lymphocytes. Normal thymus, malignant thymus [murine] and a leukemic T-cell line were compared by proton NMR spin echo experiments, and the normal thymus was found to have differed. Cells transformed by the Epstein-Barr virus can also be characterized and shown to differ from the leukemically transformed cells by spin echo experiments. Since no probe molecule was required to obtain these results, this is the 1st definitive evidence that the structure and fluidity of the plasma membranes change as a result of transformation of lymphocytes. Proton NMR spectroscopy can now be used to compare the effect of different mitogens on T- and B-lymphocytes as well as to monitor the effects of drugs, metals, etc., on the plasma membrane of transformed lymphocytes.