Human Rhinovirus 2A Proteinase Cleavage Sites in Eukaryotic Initiation Factors (eIF) 4GI and eIF4GII Are Different
- 15 April 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (8), 5026-5029
- https://doi.org/10.1128/jvi.77.8.5026-5029.2003
Abstract
Several picornaviruses shut down host cellular protein synthesis by proteolytic cleavage of the eukaryotic initiation factor (eIF) 4GI and eIF4GII isoforms. Viral RNA translation is maintained by a cap-independent mechanism. Here, we identify the human rhinovirus 2 2A pro cleavage site in eIF4GII in vitro as PLLNV 699 *GSR; this sequence lies seven amino acids C-terminal to the cleavage site previously identified in eIF4GI (LSTR681*GPP).Keywords
This publication has 29 references indexed in Scilit:
- Generation of Multiple Isoforms of Eukaryotic Translation Initiation Factor 4GI by Use of Alternate Translation Initiation CodonsMolecular and Cellular Biology, 2002
- Internal ribosome entry sites in eukaryotic mRNA moleculesGenes & Development, 2001
- Structure of translation factor elF4E bound to m7GDP and interaction with 4E-binding proteinNature Structural & Molecular Biology, 1997
- Mapping of Functional Domains in Eukaryotic Protein Synthesis Initiation Factor 4G (eIF4G) with Picornaviral ProteasesJournal of Biological Chemistry, 1995
- Purification of two picornaviral 2A proteinases: Interaction with eIF-4.gamma. and influence on the in vitro translationBiochemistry, 1993
- Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNANature, 1988
- Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.Proceedings of the National Academy of Sciences, 1979
- Capping of eucaryotic mRNAsCell, 1976
- The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.Proceedings of the National Academy of Sciences, 1976
- 5'-terminal structure of poliovirus polyribosomal RNA is pUp.Proceedings of the National Academy of Sciences, 1976