A survey of expressed genes in Caenorhabditis elegans
- 1 May 1992
- journal article
- Published by Springer Nature in Nature Genetics
- Vol. 1 (2), 114-123
- https://doi.org/10.1038/ng0592-114
Abstract
As an adjunct to the genomic sequencing of Caenorhabditis elegans, we have investigated a representative cDNA library of 1,517 clones. A single sequence read has been obtained from the 5' end of each clone, allowing its characterization with respect to the public databases, and the clones are being localized on the genome map. The result is the identification of about 1,200 of the estimated 15,000 genes of C. elegans. More than 30% of the inferred protein sequences have significant similarity to existing sequences in the databases, providing a route towards in vivo analysis of known genes in the nematode. These clones also provide material for assessing the accuracy of predicted exons and splicing patterns and will lead to a more accurate estimate of the total number of genes in the organism than has hitherto been available.Keywords
This publication has 24 references indexed in Scilit:
- Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologuesNature Genetics, 1992
- The C. elegans genome sequencing project: a beginningNature, 1992
- New yeast actin-like gene required late in the cell cycleNature, 1992
- Linear amplification sequencing, a powerful method for sequencing DNAMethods, 1991
- YACs and the C. elegans genomeBioEssays, 1991
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- Basic local alignment search toolJournal of Molecular Biology, 1990
- The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic libraryCell, 1987
- A trans-spliced leader sequence on actin mRNA in C. elegansCell, 1987
- Preparation of large numbers of plasmid DNA samples in microtiter plates by the alkaline lysis methodGene Analysis Techniques, 1987