Chemisorbed poly(propylene sulphide)-based copolymers resist biomolecular interactions
- 23 March 2003
- journal article
- research article
- Published by Springer Nature in Nature Materials
- Vol. 2 (4), 259-264
- https://doi.org/10.1038/nmat851
Abstract
Alkanethiolates have been widely used as chemisorbates to modify gold surfaces, in spite of their relatively poor oxidative stability. We introduce gold-chemisorbing block copolymers bearing an anchoring block of poly(propylene sulphide) (PPS), selected in the expectation of greater stability. These materials offer a more robust approach to surface modification of gold. As an example, a triblock copolymer with poly(ethylene glycol) (PEG) was selected, with the goal of minimizing biological adsorption and adhesion. The copolymer PEG17-bl-PPS25-bl-PEG9 chemisorbed to form a dense monolayer of 226 ± 26 ng cm−2, ∼2.2 nm thick. The copolymeric adlayer was much more stable to oxidation than commonly used alkanethiolates. Its presence greatly reduced protein adsorption (>95%), even after exposure to whole blood serum (>55 mg ml−1), as well as cell adhesion over long culture durations (>97%). PPS-containing copolymers are an attractive alternative to alkanethiolates, and PEG-bl-PPS-bl-PEG presents a powerful example for use in biodiagnostic and bioanalytical devices.Keywords
This publication has 38 references indexed in Scilit:
- Using Microcontact Printing to Pattern the Attachment of Mammalian Cells to Self-Assembled Monolayers of Alkanethiolates on Transparent Films of Gold and SilverExperimental Cell Research, 1997
- Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold.Proceedings of the National Academy of Sciences, 1996
- Comparison of the structures and wetting properties of self-assembled monolayers of n-alkanethiols on the coinage metal surfaces, copper, silver, and goldJournal of the American Chemical Society, 1991
- Wet chemical approaches to the characterization of organic surfaces: self-assembled monolayers, wetting, and the physical-organic chemistry of the solid-liquid interfaceLangmuir, 1990
- Fundamental studies of microscopic wetting on organic surfaces. 1. Formation and structural characterization of a self-consistent series of polyfunctional organic monolayersJournal of the American Chemical Society, 1990
- Molecular order at the surface of an organic monolayer studied by low energy helium diffractionThe Journal of Chemical Physics, 1989
- Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto goldJournal of the American Chemical Society, 1989
- Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy, and electrochemistryJournal of the American Chemical Society, 1987
- Fundamental studies of the chemisorption of organosulfur compounds on gold(111). Implications for molecular self-assembly on gold surfacesJournal of the American Chemical Society, 1987
- Adsorption of bifunctional organic disulfides on gold surfacesJournal of the American Chemical Society, 1983