Preliminary extraction of gonadotrophic principle from pregnant mare serum

Abstract

A method is described by which a purified fraction of gonadotropic hormone may be obtained from pregnant mare serum, the essential steps being the precipitation of the inert serum proteins by a suitable conc. of sulfo-salicylic acid, and subsequent purification by dialysis and fractional precipitation with acetone. The material obtained was a white powder readily soluble in water, and represented approximately a 1000-fold purification of the hormone in terms of solid content of original serum.