Methylation of Mouse Liver DNA Studied by Means of the Restriction Enzymes Msp I and Hpa II
- 9 March 1979
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 203 (4384), 1019-1021
- https://doi.org/10.1126/science.424726
Abstract
The restriction enzymes Hpa II and Msp I both recognize the sequence 5'-CCGG (C, cytosine; G, guanine). However, Hpa II cuts mouse liver DNA to fragments four times larger than does Msp I. The size of DNA cut by Msp I is close to that predicted from base composition and nearest neighbor analysis. The most probable explanation of these results is that in mouse the site 5'-CCGG is highly methylated.Keywords
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