IMMUNOGLOBULIN SYNTHESIS AND SECRETION, V. INCORPORATION OF LEUCINE AND GLUCOSAMINE INTO IMMUNOGLOBULIN ON FREE AND BOUND POLYRIBOSOMES
- 1 August 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 66 (4), 1183-1189
- https://doi.org/10.1073/pnas.66.4.1183
Abstract
Mouse myeloma cells pulse-labeled in vitro with (3)H-leucine or (3)H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.Keywords
This publication has 28 references indexed in Scilit:
- ULTRASTRUCTURAL LOCALIZATION OF ANTIBODY IN DIFFERENTIATING PLASMA CELLSThe Journal of Experimental Medicine, 1968
- Ribosomes in rat liver: An estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivoJournal of Molecular Biology, 1967
- Studies on free and membrane-bound ribosomes in rat liverJournal of Molecular Biology, 1967
- Biosynthesis of immunoglobulins: The separate classes of polyribosomes synthesizing heavy and light chainsJournal of Molecular Biology, 1967
- The synthesis and storage of protein by isolated lymphoid cells, examined by autoradiography with the electron microscopeJournal of Anatomy, 1966
- Polyribosomal synthesis and assembly of the H and L chains of gamma globulin.Proceedings of the National Academy of Sciences, 1966
- Synthesis and transfer of amylase in pigeon pancreatic micromosomes.1966
- BIOSYNTHESIS OF GLYCOPROTEIN BY LIVER - INCORPORATION IN VIVO OF 14C-GLUCOSAMINE INTO PROTEIN-BOUND HEXOSAMINE AND SIALIC ACID OF RAT LIVER SUBCELLULAR FRACTIONS1966
- On the biological role of glycoproteinsJournal of Theoretical Biology, 1966
- Studies on the protein-synthesizing activity of the ribosomes of rat liver. The activity of free polysomesBiochemical Journal, 1965