A Restriction Map of Xenopus laevis Mitochondrial DNA
Open Access
- 1 August 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 126 (1), 119-127
- https://doi.org/10.1111/j.1432-1033.1982.tb06755.x
Abstract
The mitochondrial DNA from X. laevis is a 17.4 .times. 103-base-pair circular DNA molecule. The mapping of this DNA, using 19 different restrictionendonucleases is reported here. The sites are as follows: 1 for BamHI, PstI, SacI, SalI, BalI; 2 for BglII, SacII, EcoRI, ClaI, 3 for XhoI, 4 for AvaI, XbaI, PvuII, 5 for HindIII, 6 for HhaI, BclI, HpaI, 10 for AvaII and 11 for HincII. The same sites (except for 1 of the 2 ClaI sites) are observed in the molecule cloned in pBR322 DNA. The fragments corresponding to 62 cleavage sites were all ordered and precisely located. They provide suitable conditions for further investigations connected with the study of replication and nucleotide sequence determination of this molecule.This publication has 25 references indexed in Scilit:
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