Mitochondrial KATP channel opening protects a human atrial-derived cell line by a mechanism involving free radical generation

Abstract

Objectives: The mechanism by which the mitochondrial K_ATP channel openers confer protection against ischemia/reperfusion injury is debated. Evidence suggests that rather than solely being an end effector, opening of these channels may act by a trigger mechanism. We examined the effects of the mitochondrial K_ATP channel opener, diazoxide on parameters of mitochondrial function with specific reference to reactive oxygen species (ROS) generation in a human atrial derived cell line model of simulated ischemia/reperfusion (LSI/R). Methods and results: Propidium iodide (PI) exclusion was used to assess survival. Diazoxide treatment conferred protection against LSI/R (13.9±0.9% vs. 36.9±4.5% controls) that was abolished by pre-treatment with the mitoK_ATP channel blocker, 5-hydroxydecanoate (5-HD) (33.3±3.6%) and with the free radical scavenger, 2-mercaptopropionylglycine (MPG) (29±4.0%). Diazoxide caused increased oxidation of the ROS probe, reduced mitotracker orange (1.3 vs. 1.0 arbitrary units for control; PConclusion: These results demonstrate for the first time that the mitoK_ATP channel opener diazoxide can act as a trigger of preconditioning by a mechanism involving mitochondrial swelling and the generation of ROS.