SYNTHESIS OF TRANSFER RNA BY ISOLATED NUCLEI

Abstract

Isolated pea nuclei contain an active transfer RNA which will accept amino acids from both cytoplasmic and nuclear amino acid activating enzymes. Isolated pea nuclei synthesize in vitro a small molecule RNA of the physical characteristics of amino acid transfer RNA. This synthesis is DNA-dependent. It has been shown that this RNA is in fact transfer RNA and that the in vitro synthesized molecules are capable of accepting amino acids.