The interrelationships of edaphic factors and distribution of soil fungi of Varanasi were studied. Water holding capacity, moisture content, pH values, total organic carbon content, total N content and total water soluble salts of 3 different soils were taken into account. Chemical analysis of soils was carried out bimonthly and the distribution of fungi was studied 3 times a month throughout the year. Moisture content and pH values of the soils have a pronounced effect on the distribution of fungi and the change in these values is accompanied by similar change in population of fungal species. Aspergillus niger, Rhizopus nigricans, R. arrhizus, Phycomyces sp. and Cunninghamella echinulata are dominant species isolated nearly throughout the year from all the plots. Eighty-seven different species of microfungi have been isolated and identified, 20 belonging to Phycomycetes, 9 to Ascomycetes, 56 to Deutermycetes and only 2 to Basidiomycetes.