New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants
- 15 July 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (14), 4386-4388
- https://doi.org/10.1128/jb.183.14.4386-4388.2001
Abstract
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.Keywords
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