Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 170 (2), 161-169
- https://doi.org/10.1007/bf00337792
Abstract
Bacteriophage lambda vectors, derived from λplac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the β-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac - and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of β-galactosidase synthesized by the vector bacteriophage. The λZEQS vector has been certified B2 (EK2) by the French control commission “Recombinaisons génétiques in vitro”.This publication has 35 references indexed in Scilit:
- Maximizing gene expression on a plasmid using recombination in vitroCell, 1978
- The rabbit β-globin gene contains a large insert in the coding sequenceCell, 1977
- Recombinant DNAAnnual Review of Biochemistry, 1977
- Improved derivative of a phage λ EK2 vector for cloning recombinant DNANature, 1976
- Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragmentsNature, 1974
- Properties of a mutant of Escherichia coli defective in bacteriophage λ head formation (groE): I. Initial characterizationJournal of Molecular Biology, 1973
- Deletion mutants of bacteriophage lambdaJournal of Molecular Biology, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The nature of mutants in the lac promoter regionJournal of Molecular Biology, 1968
- Direction of Transcription of a Regulatory Gene in E. coliNature, 1968