Commercial Recombinant Human β‐Nerve Growth Factor and Adult Rat Dorsal Root Ganglia Contain an Identical Molecular Species of Nerve Growth Factor Prohormone
- 1 May 2000
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 74 (5), 2127-2133
- https://doi.org/10.1046/j.1471-4159.2000.0742127.x
Abstract
Examination of commercial recombinant human β-nerve growth factor (rh-β-NGF) preparations with polyclonal antibodies specific to 13-kDa NGF and pro-NGF-specific domains revealed the presence of high-molecular-mass immunoreactive proteins, including a 60-kDa NGF prohormone. On incubation with a mixture of N- and O-specific glycosidases, the 60-kDa NGF prohormone generated a 32-kDa protein corresponding to the molecular size of NGF precursor predicted by the cloned human NGF cDNA. Highly sensitive chemiluminescence immunoblot analysis of adult rat dorsal root ganglia, spinal cord, and colon tissues with NGF- and pro-NGF domain-specific antibodies also revealed the presence of high-molecular-mass proteins, including the 60-kDa NGF prohormone. Based on the presence of the 60-kDa NGF prohormone in dorsal root ganglia and its efferent tissues, we suggest that proteolytically unprocessed, glycosylated NGF prohormone may mediate interactions between neurons and the tissues they innervate.Keywords
This publication has 21 references indexed in Scilit:
- Nerve growth factor and epidermal growth factor in mouse submandibular glands: identical diurnal changes and rates of secretagogue-induced synthesisEndocrinology, 1993
- Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.The Journal of cell biology, 1990
- Interaction of antibodies to synthetic peptides of proNGF with in vitro synthesized NGF precursorsFEBS Letters, 1989
- Co-localization of the nerve growth factor precursor protein and mRNA in the mouse submandibular glandNeuroscience Letters, 1988
- Nerve growth factor: biosynthetic products of the mouse salivary glands. Characterization of stable high molecular weight and 32,000-dalton nerve growth factorsBiochemistry, 1986
- Differential RNA splicing predicts two distinct nerve growth factor precursorsNature, 1986
- Human β-nerve growth factor gene sequence highly homologous to that of mouseNature, 1983
- Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factorNature, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970