THE NUTRITION OF ANIMAL TISSUES CULTIVATED IN VITRO
Open Access
- 25 March 1957
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 3 (2), 141-150
- https://doi.org/10.1083/jcb.3.2.141
Abstract
1. The amino acid requirements of freshly explanted chick embryonic heart tissues cultivated in completely synthetic media have been determined, employing a nutritional depletion technique. Arginine, histidine, lysine, tyrosine, tryptophan, phenylalanine, cystine, methionine, threonine, leucine, and valine were found to be essential. Serine, isoleucine, glycine, and glutamine were found to be non-essential. Glutamic acid, aspartic acid, α-alanine, proline, and hydroxyproline were found to be inhibitory in this test system.Keywords
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