Survival of Spermatozoa in a Human Sperm Bank
- 12 February 1973
- journal article
- research article
- Published by American Medical Association (AMA)
- Vol. 223 (7), 774-777
- https://doi.org/10.1001/jama.1973.03220070030007
Abstract
Human semen was frozen and stored in liquid nitrogen for periods of time up to eight years and two months. A total of 1,764 vials containing 1.1 ml of semen from 533 ejaculates provided by 207 donors were frozen. The freezing and thawing process induced a loss of approximately 50% of the original motility. Storage in liquid nitrogen for periods up to 36 months resulted in no further significant loss in mean motility. However, after this time, a progressive significant loss in motility was observed throughout the period of this study. Specimens with initial sperm counts between 40 and 200 million/ml and initial motilities between 60% and 89% appeared to withstand long-term storage best.Keywords
This publication has 8 references indexed in Scilit:
- Frozen human semen for artificial inseminationAmerican Journal of Obstetrics and Gynecology, 1969
- Heterologous and Homologous Inseminations with Human Semen Frozen and Stored in a Liquid-Nitrogen RefrigeratorFertility and Sterility, 1966
- FACTORS AFFECTING THE DILUTION, FREEZING AND STORAGE OF HUMAN SEMENReproduction, 1966
- Conception with Human Spermatozoa Frozen by Nitrogen Vapor TechnicFertility and Sterility, 1964
- Improved Methods of Preservation of Human Spermatozoa by Freezing and Freeze-DryingFertility and Sterility, 1963
- Artificial Insemination of CattleJournal of Dairy Science, 1961
- Fertilizing Capacity of Frozen Human SpermatozoaNature, 1953
- Revival of Spermatozoa after Vitrification and Dehydration at Low TemperaturesNature, 1949