Regulatory mutants and transcriptional control of the Serratia marcescens extracellular nuclease gene
- 1 March 1992
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (5), 643-651
- https://doi.org/10.1111/j.1365-2958.1992.tb01512.x
Abstract
The extracellular nuclease of Serratia marcescens is regulated in a complex fashion. Unlike most catabolic enzymes, it appears not to be substrate regulated. However we have shown it to be regulated by an SOS-like system in S. marcescens. Additionally nuclease expression is regulated in a growth-phase-dependent manner. In this work we demonstrate that growth-phase-dependent regulation is at the transcriptional level. The putative LexA-binding site which mediates SOS regulation is shown to act as an operator site in vivo. The boundaries of a minimal promoter, still regulated by growth phase and SOS regulation, are defined along with the transcriptional start site. However, a region upstream of the nuclease promoter is shown to enhance significantly the expression of nuclease.Keywords
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