Ribosomal Subunit Localization of Hemoglobin Messenger RNA
Open Access
- 1 May 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 54 (1), 75-80
- https://doi.org/10.1111/j.1432-1033.1975.tb04115.x
Abstract
When rabbit reticulocyte polyribosomes are treated with 0.5 M KCl, they dissociate into subunits and release a protein fraction which is required for peptide chain initiation in a cell-free system using KCl-treated subunits as the source of ribosomes. Three independent methods were used to determine the fate of mRNA after KCl treatment of the subunits. These three methods (sucrose gradient analysis of RNA after dissociating it from protein with sodium dodecylsulfate, acrylamide gel electrophoresis of RNA and electron microscopic analysis of subunits) all showed the 8--9-S mRNA to be associated with the small subunit, but not the large subunit. Furthermore, no mRNA was found to be associated with either "native" ribosomal subunit in a reticulocyte lysate.Keywords
This publication has 29 references indexed in Scilit:
- Presence of haemoglobin messenger RNA in the postribosomal supernatant of rabbit reticulocytes and conditions necessary for its translationFEBS Letters, 1972
- Conservation of globin messenger RNA in rabbit reticulocyte monoribosomes after sodium fluoride treatmentBiochemical and Biophysical Research Communications, 1972
- Involvement of hemin, a stimulatory fraction from ribosomes and a protein synthesis inhibitor in the regulation of hemoglobin synthesisJournal of Molecular Biology, 1972
- Interaction of haemoglobin messenger RNP with the small subparticle of reticulocyte ribosomesFEBS Letters, 1970
- Electron microscopic studies on the biosynthesis of the 50 s ribosomal subunit in Escherichia coliJournal of Molecular Biology, 1970
- Effects of cycloheximide on polyribosome function in reticulocytesJournal of Molecular Biology, 1967
- The dissociation of reticulocyte polysomes into subunits and the location of messenger RNAJournal of Molecular Biology, 1966
- Studies on the genesis of polyribosomes: II. The association of nascent messenger RNA with the 40 s subribosomal particleJournal of Molecular Biology, 1965
- Polypeptide synthesis in Escherichia coli: II. The polypeptide chain and S-RNAJournal of Molecular Biology, 1963
- Controlled dissociation of rabbit reticulocyte ribosomes and its effect on hemoglobin synthesisJournal of Molecular Biology, 1962