Scanning and Transmission Electron Microscopy of the Normal Rabbit Aortic Endothelium after Controlled Perfusion Fixation
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in Cells Tissues Organs
- Vol. 112 (1), 79-91
- https://doi.org/10.1159/000145499
Abstract
Light microscopy, scanning electron microscopy, and transmission electron microscopy were employed on the anterior wall of the rabbit thoracic aorta with the particular purpose to study endothelial marginal flaps and the structure of stomata under physiological conditions at 100 mm Hg. Intra-aortic pressure monitoring was performed during the whole period of perfusion (2 h), restricting pressure variations to a maximum of ± 5 mm Hg in order to preserve endothelial architecture. We found the endothelial surface uniformly smooth. Marginal flaps of the endothelial cells were mutually overlapping, often forming multiple layers. Underlying flaps might protrude to the lumen between neighbouring flaps as flat mushrooms. Flaps of adjacent cells might diverge for short distances to decover an underlying endothelial cell. Downstream flaps of the cells might be overlapped at their base by flaps of adjacent cells, the extremity of the flap thus appearing as a disc separated from the cell. The small discs and troughs thus formed are considered equivalent to the so-called stomata.Keywords
This publication has 11 references indexed in Scilit:
- Quantitative study of the rabbit aortic endothelium using vascular castsAtherosclerosis, 1980
- Arterial endothelium around rabbit aortic ostia: A SEM study using vascular castsExperimental and Molecular Pathology, 1979
- Cellular pathology of the rat aortaVirchows Archiv, 1979
- Focal “Spontaneous” Alterations and Loss of Endothelial Cells in Rabbit AortaActa Pathologica Microbiologica Scandinavica Section A Pathology, 1978
- Scanning electron microscopy of arteries The morphology of aortic endothelium in haemodynamically stressed areas associated with branchesAtherosclerosis, 1977
- ENDOTHELIAL STRUCTURE IN RABBITS WITH MODERATE HYPERCHOLESTEROLEMIA - SCANNING ELECTRON-MICROSCOPIC STUDY1977
- Observations on the microcirculatory bed in rat mesocecum using differential interference contrast microscopy in vivo and electron microscopyJournal of Anatomy, 1976
- Regional variation in rat aortic endothelial surface morphology: Relationship to regional aortic permeabilityExperimental and Molecular Pathology, 1976
- Preparation of vascular endothelium for scanning electron microscopy: a comparison of the effects of perfusion and immersion fixationJournal of Microscopy, 1975
- Histochemical investigations on the nature of large blood vessel endothelial and medial argyrophilic lines and on the mechanism of silver stainingHistochemistry and Cell Biology, 1968