Decreased Cloning of Lymphocytes from Elderly Individuals
- 1 April 1984
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 19 (4), 293-296
- https://doi.org/10.1111/j.1365-3083.1984.tb00933.x
Abstract
Limiting-dilution cloning of human lymphocytes was used to study cloning and proliferation of lymphocytes in 10 young and 10 elderly individuals. The cloning efficiency for elderly individuals, 3.5%, was significantly lower than that for young individuals, 19.8%. Mixing experiments showed that this decrease was due to both a decrease in the frequency of the clone-forming cells and a decrease in the interacting ability of the lymphocytes. Lymphocytes from elderly individuals which did form clones proliferated at the same rate as did those from young individuals.This publication has 14 references indexed in Scilit:
- Direct demonstration of the clonogenic potential of every human peripheral blood T cell. Clonal analysis of HLA-DR expression and cytolytic activity.The Journal of Experimental Medicine, 1983
- T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.Proceedings of the National Academy of Sciences, 1982
- Semi-automated limit-dilution assay and clonal expansion of all T-cell precursors of cytotoxic lymphocytesJournal of Immunological Methods, 1982
- Immunological studies of aging. Decreased production of and response to T cell growth factor by lymphocytes from aged humans.Journal of Clinical Investigation, 1981
- Cytokinetic analysis of the impaired proliferative response of peripheral lymphocytes from aged humans to phytohemagglutinin.The Journal of Experimental Medicine, 1979
- Lymphocyte subsets and integrated immune function in aging humansClinical Immunology and Immunopathology, 1978
- Immunological studies of Aging. III. Cytokinetic basis for the impaired response of lymphocytes from aged humans to plant lectins.The Journal of Experimental Medicine, 1977
- AGE DEPENDENCE OF T LYMPHOCYTESThe Lancet, 1976
- Impaired Lymphocyte Function in Aged HumansJournal of Clinical Investigation, 1974
- Mitogenic Effect of Phytohaemagglutinin at Different AgesNature, 1967