Propagation of Measles Virus in Suckling Mice.

Abstract

Summary HeLa adapted measles virus (Edmonston strain) was propagated in suckling mice through 24 serial intracerebral passages. The virus produced a lethal infection in most animals 7 to 13 days following inoculation. Identity of mouse propagated agent was established by serum neutralization tests in tissue culture and in suckling mice. Further identification was established by protection of suckling mice born of mothers immunized with tissue culture measles virus. Fluid phase from tissue cultures inoculated with late mouse passage materials did not contain infectious material either for HeLa cells or for suckling mice. Infections, however, were readily induced when cells were employed as inoculum.