Phospholipids in Human Young Placenta*

Abstract

Phospholipids of human young placenta were separated by the silicic acid-Hyflo Super-Cel (21) column chromatography and identified with thin layer chromatography. Further identification was carried out with the mild alkali hydrolysate using paper and thin layer chromatography. Cardiolipin, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylcholine, lysolecithin and sphingomyelin were observed in human young placenta. The composition of fatty acids of these phospholipids were also analyzed. p32 was incorporated into cardiolipin, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylcholine, lysolecithin and sphingomyelin in the tissue slice experiment of human young placenta. A relatively large amount of p32 was incorporated into sphingomyelin, phosphatidylcholine and phosphatidylserine. Estradiol stimulated the incorporation of p32 into phosphatidylethanolamine markedly.