Séparation des particules ribonucléoprotéiques (Hn‐RNP) des noyaux de cellules L, en deux classes, par centrifugation isopycnique en gradients de métrizamide
- 1 August 1975
- journal article
- Published by Wiley in FEBS Letters
- Vol. 56 (2), 341-347
- https://doi.org/10.1016/0014-5793(75)81123-9
Abstract
Separation of nuclear Hn-RNP particles into two density classes, following isopycnic centrifugation in metrizamide gradients, is reported. One class (density 1.31 g/ml) is rapidly labelled, and contains polydisperse heterogeneous high molecular weight particles, easily diffusible from intact nuclei under certain conditions. The other class (density 1.18 g/ml) is of lower molecular weight, not diffusible, and needs apparently a longer time to be labelled and/or to accumulate inside the nuclei.Keywords
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