Method for Study of Electrocardiogram of Early Chick Embryo within the Shell

Abstract
A simple technique of obtaining ecg of early chick embryos without removal from the shell is described. Rhode Island chick embryos, 3-16 days old, were mounted in a metal egg holder with a circulating water jacket. The recording electrodes were applied with the aid of a dissecting microscope. Records were made on a Grass III D ecg with an EKG filter. Examples are given of records obtained at various temperatures before and after injection of different drugs.