The Isolation of Plant Cuticle with Pectic Enzymes.
- 1 January 1955
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 30 (1), 78-80
- https://doi.org/10.1104/pp.30.1.78
Abstract
Cuticle was isolated by infiltrating leaf disks with a 2 - 3% (w/v) solution of Pectinase (Nutritional Biochemicals Corp) or Enzyme 19 (Rohm and Haas Co.) buffered to pH 3.5 - 4.5 and containing 100 ppm. Merthiolate (Thimerosal, Lilly). Gentle rotary agitation at 35[degree]C resulted in a suspension of isolated cells, vascular strands, and cuticle disks. Responses of various spp. and some properties of the cuticle disks are descr.This publication has 7 references indexed in Scilit:
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