ovo/svb integrates Wingless and DER pathways to control epidermis differentiation

Abstract

In Drosophila, as in mammals, epidermal differentiation is controlled by signalling cascades¹ that include Wnt proteins²,³ and the ovo/shavenbaby (svb) family of zinc-finger transcription factors^4,5,6. Ovo/svb is a complex gene with two genetic functions corresponding to separate control regions: ovo is required for female germline development and svb for epidermal morphogenesis⁷,⁸. In the Drosophila embryo, the ventral epidermis consists of the segmental alternance of two major cell types that produce either naked cuticle or cytoplasmic extrusions known as denticles. Wingless signalling specifies smooth cells that produce naked cuticle⁹, whereas the activation of the Drosophila epidermal growth factor (EGF) receptor (DER) leads to the production of denticles¹⁰. Here we show that expression of the ovo/svb gene controls the choice between these cell fates. We find that svb is a key selector gene that, cell autonomously, directs cytoskeletal modifications producing the denticle. The DER pathway promotes denticle formation by activating svb expression. Conversely, Wingless promotes the smooth cell fate through the transcriptional repression of svb bythe bipartite nuclear factor Armadillo/dTcf. Our data indicatethat transcriptional regulation of svb integrates inputs fromthe Wingless and DER pathways and controls epidermal differentiation.