In vitro Inhibition of Radiophosphate Uptake and Growth of a Neurotropic Virus by 5-Chlorouridine.

Abstract

Minced brain from 1-day-old mice was incubated with tracer amt. of radioactive phosphate and the incorporation of radioactivity in the phospholipids, pentose nucleic acids, desoxypentose nucleic acids, and phosphoprotein fractions detd. Infection of this tissue with Theiler''s GD VII virus increased the extent of uptake by the phospholipid and pentose nucleic acids but had no influence on the other phosphorus-containing fractions. 5-chlorouridine (1 mg./ml.) inhibited the propagation of the virus and the incorporation of P32O4 into the pentosenucleic acids. Uridine partially prevented the effect of 5-chlorouridine.