Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2), 794-798
- https://doi.org/10.1073/pnas.75.2.794
Abstract
Alkali-labile lesions introduced into T7 DNA by treatment with methyl methanesulfonate were removed and the DNA was repaired by incubation with DNA polymerase alpha and nuclease from a human lymphoblastoid line followed by the addition of DNA ligase. The nuclease preparation contains both apurinic endonuclease and 5'-3' exonuclease activities. Dinucleotides appear to be the first product of exonuclease action. Repair of methyl methanesulfonate-induced damage can occur by the insertion of only a few nucleotides per lesion as in vivo.Keywords
This publication has 32 references indexed in Scilit:
- Intermediates in excision repair by human cells: use of S1 nuclease and benzoylated naphthoylated cellulose to reveal single-strand breaksBiochemistry, 1977
- Relation between Escherichia coli endonucleases specific for apurinic sites in DNA and exonuclease IIINucleic Acids Research, 1977
- Endonuclease II, apurinic acid endonuclease, and exonuclease III.Proceedings of the National Academy of Sciences, 1976
- Vertebrate DNA polymerasesCell, 1975
- Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.1974
- In Vitro Repair of Apurinic Sites in DNAProceedings of the National Academy of Sciences, 1974
- Unique Double-Stranded Fragments of Bacteriophage T5 DNA Resulting from Preferential Shear-Induced Breakage at NicksProceedings of the National Academy of Sciences, 1974
- Polynucleotides. CXXIII. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymeraseBiochemistry, 1973
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965
- The physical properties of T7 bacteriophageJournal of Molecular Biology, 1962