Crystallization and preliminary X‐ray analysis of the 9 kDa protein of the mouse signal recognition particle and the selenomethionyl‐SRP9
- 22 April 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 384 (3), 219-221
- https://doi.org/10.1016/0014-5793(96)00316-x
Abstract
Two different crystal forms of the 9 kDa protein of the signal recognition particle (SRP9) have been prepared by the hanging drop vapor diffusion technique using 28% (w/v) PEG8000 or 28% saturated ammonium sulphate as precipitant. The crystals are hexagonal bipyramids with average dimensions of 0.2 × 0.1 × 0.1 mm3 and they diffract to a resolution of 2.3 Å. They belong to the space groups P6222/P6422 or P3121/P3221 with cell dimensions a = b = 63.0 Å, and c = 111.5 Å. Crystals have also been grown from the selenomethionyl protein and multiwavelength data sets have been collectedKeywords
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