SMALL BOWEL TRANSPLANTATION IN THE RAT
- 1 June 1988
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 45 (6), 1021-1026
- https://doi.org/10.1097/00007890-198806000-00005
Abstract
We investigated the effect of pretransplant conditioning as a way to reduce the need for the aggressive immunosuppressive therapy reportedly required in small bowel (SB) allograft recipients. LEW rats were conditioned with (1) a donor-specific blood transfusion (DST) on day −8 and a concurrent 5-day course of CsA (10 mg/kg/day); (2) a nonspecific blood transfusion and CsA; (3) CsA alone. A 10-cm segment of the host native bowel was then replaced with an equivalent segment of SB obtained from ACI rats. Postoperative treatment consisted of CsA at 2.5 mg/kg/day for 30 days. Rats conditioned with a nonspecific transfusion and CsA or with CsA alone survived for 14.1±5.8 and 18.3±5.7 days, respectively. In contrast, rats conditioned with DST and CsA survived for 60.3±36.2 days (P<0.001 vs. both controls). Biopsies taken from long-term survivors showed a normal bowel architecture. The function of the allografts was studied in a group of animals totally deprived of their native bowel and transplanted with a 30-cm segment of ACI SB. CsA-DST-treated recipients survived an average of 90±43 days and grew at a rate comparable to isografted animals. Treated allograft recipients had maltose absorption indistinguishable from isografted controls at all times tested. In contrast, maltose absorption was severely impaired in recipients rejecting their grafts. This study demonstrates that long-term survival of SB allograft recipients can be achieved with good functional results with low doses of CsA in recipients conditioned with DST and CsA.This publication has 7 references indexed in Scilit:
- LONG-TERM PROLONGATION OF CARDIAC ALLOGRAFTS BY SUBTHERAPEUTIC LEVELS OF CYCLOSPORINE IN RATS CONDITIONED WITH PRETRANSPLANT BLOOD TRANSFUSIONS AND CYCLOSPORINE1985
- SUCCESSFUL SMALL BOWEL ALLOTRANSPLANTATION IN DOGS WITH CYCLOSPORINE AND PREDNISONETransplantation, 1984
- PROLONGATION OF RAT KIDNEY ALLOGRAFTS BY PRETRANSPLANT ADMINISTRATION OF DONOR ANTIGEN EXTRACT OR WHOLE BLOOD TRANSFUSION COMBINED WITH A SHORT COURSE OF CYCLOSPORINE1Transplantation, 1983
- COMPARATIVE ANALYSIS OF THE DST AND IMURAN-PLUSDST PROTOCOLS FOR LIVE DONOR RENAL TRANSPLANTATION1Transplantation, 1983
- SMALL BOWEL TRANSPLANTATION IN THE DOG USING CYCLOSPORINETransplantation, 1983
- CYCLOSPORIN A SPARES SELECTIVELY LYMPHOCYTES WITH DONOR-SPECIFIC SUPPRESSOR CHARACTERISTICSTransplantation, 1981
- PROLONGATION OF RENAL ALLOGRAFT SURVIVAL IN THE RAT BY PRETREATMENT WITH DONOR ANTIGEN AND CYCLOSPORIN ATransplantation, 1981