FSH Receptor Autoregulation and Cyclic AMP Production in the Immature Rat Testis
- 1 October 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 23 (4), 810-814
- https://doi.org/10.1095/biolreprod23.4.810
Abstract
Intratesticular injection of 3 µg ovine FSH or 1 µg rat FSH caused a 50% decrease in the number of FSH binding sites available 24 h after injection in immature rats aged 16-17 days. Measurement of injected hormone bound in vivo, by radioimmunoassay of FSH released from testes incubated at 65°C for 30 min in vitro, showed that this effect was not due to receptor occupation. FSH-sensitive cyclic AMP (cAMP) production in whole cells showed a similar decrease 24 h after FSH injection. In cell-free homogenate of tubular cells isolated from testes injected 24 h earlier with FSH, there was a reduction in the Vmax of FSH-sensitive adenylate cyclase with no change in Km.This publication has 2 references indexed in Scilit:
- Hormonal regulation of testicular luteinizing hormone receptors. Effects on cyclic AMP and testosterone responses in isolated Leydig cells.Journal of Biological Chemistry, 1977
- Gonadotropin-induced regulation of luteinizing hormone receptors and desensitization of testicular 3':5'-cyclic AMP and testosterone responses.Proceedings of the National Academy of Sciences, 1977