Determination of 2'-acetyl erythromycin and erythromycin in human tonsil tissue by HPLC with coulometric detection
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 21 (suppl D), 67-72
- https://doi.org/10.1093/jac/21.suppl_d.67
Abstract
A simple and sensitive high-performance liquid chromatographic method was developed for the determination of 2′-acetyl erythromycin and erythromycin in human tonsil tissue. Methyl tert-butyl ether was used as the extraction solvent after alkalization of tissue homogenates. Separation was achieved on a reverse phase C-18 column. The mobile phase consisted of acetonitrile-methanol-tetrahydrofuran-sodium acetate buffer, pH 4.5. Eluted compounds were monitored by a coulometric detector in the oxidativc screen mode. The quantitation limits of 2′-acetyl erythromycin and erythromycin were 0.20 and 0.30 mg/kg of tissue, respectively. The method was linear over the concentration range of 0.60–10.0 mg/kg of tissue for 2′-acetyl erythromycin and erythromycin.Keywords
This publication has 1 reference indexed in Scilit:
- Assay of Erythromycin from Human Serum by High Performance Liquid Chromatography with Electrochemical DetectionJournal of Liquid Chromatography, 1984