Protein kinase C phosphorylates DNA topoisomerase I
- 18 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 259 (1), 57-60
- https://doi.org/10.1016/0014-5793(89)81493-0
Abstract
The induction of mammalian cell proliferation requires the expression of a specific set of genes. Tumor promoters stimulate cell growth by activating the Ca2+ and phospholipid-dependent protein kinase, protein kinase C (PKC). DNA topoisomerase I, a nuclear enzyme involved in transcription, was phosphorylated by activated PKC in vitro. Phosphorylation by PKC stimulated the DNA relaxation activity of topoisomerase I two- to three-fold. Therefore, DNA topoisomerase I is a substrate for PKC-mediated activation by phosphorylation and may serve as a nuclear target of mitogenic signals generated by tumor promoters in vivo.Keywords
This publication has 60 references indexed in Scilit:
- Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREBNature, 1988
- Characterization of ribosomal RNA synthesis in a gene dosage mutant: the relationship of topoisomerase I and chromatin structure to transcriptional activity.The Journal of cell biology, 1987
- Protein kinase C phosphorylates leukemia RNA polymerase IIBiochemical and Biophysical Research Communications, 1987
- Immunochemical characterization of protein kinase C in rat liver nuclei and subnuclear fractionsBiochemical and Biophysical Research Communications, 1986
- Inhibition of topoisomerase I by antibodies in sera from scleroderma patientsFEBS Letters, 1986
- Selective phosphorylation of human DNA methyltransferase by protein kinase CFEBS Letters, 1986
- DNA topoisomerase I and II activities during cell proliferation and the cell cycle in cultured mouse embryo fibroblast (C3H 10T1/2) cellsExperimental Cell Research, 1985
- Phosphorylation of purified Novikoff hepatoma topoisomerase IBiochemical and Biophysical Research Communications, 1983
- Purification of a protein kinase from human Namalwa cells that phosphorylates topoisomerase IBiochemical and Biophysical Research Communications, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970