A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome
- 1 September 1995
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 377 (6547), 309-314
- https://doi.org/10.1038/377309a0
Abstract
Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson–Crick G–C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.Keywords
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