Release by human chromosome 3 of the block at G1 of the cell cycle, in hybrids between tsAF8 hamster and human cells.
- 1 June 1976
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (6), 2052-2055
- https://doi.org/10.1073/pnas.73.6.2052
Abstract
A temperature-sensitive mutant of Syrian hamster cells, AF8 was fused with simian-virus-40-transformed Lesch-Nyhan fibroblasts; LNSV, in the presence of beta-propiolactone-inactivated Sendai virus. The AF8 cells grow well at 33.5 degrees but are arrested in mid G1 period when shifted to 39 degrees. The LNSV cells are deficient in hypoxanthine guanine phosphoribosyltransferase. The hybrid clones were selected in hypoxanthine-aminopterin-thymidine medium at 39 degrees. A total of 20 clones was isolated and karyotyped. All clones contained most of the hamster chromosomes and one to eight human chromosomes. The preferential retention of human chromosome 3 was observed in 100% of the metaphases of all clones. In nine of the clones, the only human chromosome present was chromosome 3. The results indicate that human chromosome 3 is responsible for conferring to the hybrid cells the ability to grow at nonpermissive temperature for AF8 cells, i.e., the ability to overcome the G1 block. These findings, together with other reports in the cell cycle may be regulated by genes located on different chromosomes.Keywords
This publication has 23 references indexed in Scilit:
- Mapping of the G1 phase of a mammalian cell cycleNature, 1975
- Reversible alterations in the mitotic cycle of chick embryo cells in various states of growth regulationJournal of Cellular Physiology, 1975
- A temperature‐sensitive cell cycle mutant of the BHK cell lineJournal of Cellular Physiology, 1974
- A mammalian somatic ?cell cycle? mutant defective in G1Journal of Cellular Physiology, 1974
- Positive Control of Transformed Phenotype in Hybrids between SV40-Transformed and Normal Human CellsScience, 1974
- Temperature-sensitive Mammalian Cell Line Blocked in MitosisNature, 1974
- DNA synthesis and mitosis in a temperature sensitive Chinese hamster cell lineJournal of Cellular Physiology, 1973
- Unidirectional loss of human chromosomes in rat-human hybridsExperimental Cell Research, 1973
- Conditionally lethal mutations in chinese hamster cells. I. Isolation of a temperature‐sensitive line and its investigation by cell cycle studiesJournal of Cellular Physiology, 1973
- A cell line which is temperature-sensitive for cytokinesisJournal of Cellular Physiology, 1972