Formation of an aspartyl phosphate intermediate in the reactions of nucleoside phosphotransferase from carrots
- 1 February 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 155 (1), 121-124
- https://doi.org/10.1111/j.1432-1033.1986.tb09466.x
Abstract
The nucleoside phosphotransferase from carrots forms N-phosphorylhydroxylamine when substrates are hydrolysed in the presence of hydroxylamine. Denaturation of the enzyme after short incubation with the substrates leads to a protein, in which, after reduction with [3H]NaCNBH3 and complete hydrolysis with 6 M HCl, labelled homoserine can be detected. The first experiment provides evidence for an activated phosphorylenzyme, the second experiment shows that the intermediate is an acyl phosphate formed by a nucleophilic attack of an aspartate .beta.-carboxylate group.This publication has 12 references indexed in Scilit:
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