Effect of Thyroid Hormone on the Ploidy of Rat Liver Nuclei as Determined by Flow-Cytometry
- 1 May 1978
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 158 (1), 63-67
- https://doi.org/10.3181/00379727-158-40140
Abstract
The ploidy distributions of rat liver nuclei isolated from normal and thyroidectomized rats were determined after conjugation with fluorochromes acridine orange and propidium iodide by rapid flow cytometry on the basis of their DNA content. Using this technique, the marked influence of thyroid status on the ploidy of rat liver nuclei as compared to euthyroid rats of the same age was confirmed. No further development of tetraploid nuclei occurred from the time of thyroidectomy. However, administration of 1 mg T3[triiodothyronine]/100 g to hypothyroid rats led within 6 days to ploidy patterns identical with those observed in euthyroid animals of the same age.This publication has 6 references indexed in Scilit:
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