The chloroplast nucleoid inOchromonas danica: I. three-dimensional morphology in light and dark-grown cells

Abstract

The 3-dimensional structure of the plastid nucleoid was determined from serial sections of the plastid of dark-grown, greening, and light-grown cells of Ochromonas danica. In light-grown and greening cells, the chloroplast nucleoid forms a continuous cord or ring which closely follows the rim of each lateral lobe of the chloroplast and is continuous across the top and bottom of the bridge connecting the 2 chloroplast lobes. The nucleoid always lies just inside the chloroplast girdle bands where they loop around the rim of the plastid. It was demonstrated by electron-microscopic autoradiography of greening cells labelled with [³H]-thymidine that all the plastid DNA is localized in this peripheral ring-shaped nucleoid. In the proplastid of dark-grown cells, the nucleoid also forms a ring-shaped structure lying just inside the single girdle thylakoid, although frequent irregularities, such as gaps, are present. It is postulated that the girdle bands determine the shape of the chloroplast nucleoid, possibly by having specific attachment sites for the plastid DNA molecules. A survey of the literature shows that a peripheral ring-shaped chloroplast nucleoid is a characteristic feature of the 5 classes of algae whose chloroplasts possess girdle bands, namely the Raphidophyceae, Chrysophyceae, Bacillariophyceae, Xanthophyceae, and Phaeophyceae, and has never been observed in plants whose plastids lack girdle bands.