IDIOTYPIC ANALYSIS OF ANTI-GAT ANTIBODIES .1. PRESENCE OF COMMON IDIOTYPIC SPECIFICITIES IN BOTH RESPONDER AND NONRESPONDER MICE

Abstract

The synthetic terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) induced anti-GAT antibodies [Ab] in D1.LP mice. Some of these Ab shared common idiotypic specificities. The interaction between idiotypic Ab and anti-idiotypic antiserum was specifically inhibited by GAT and the closely related polymer of L-glutamic acid and L-tyrosine (GT) but not by the copolymer of L-glutamic acid and L-alanine (GA). The binding of [radioactively] labeled GAT by the anti-GAT Ab was specifically inhibited by the anti-idiotypic antiserum. The results indicated a close association of idiotypic determinants with the Ab-combining sites. Such active site-related common idiotypic specificities were found in individual sera of responder mice (D1.LP) and nonresponder mice (DBA/1) immunized with GAT and GAT-MBSA [methylated bovine serum albumin], respectively, indicating that responder and nonresponder mice carry B [bone marrow-derived] cells with common clonotypes.