Bioorthogonal Ligation in the Spotlight
- 17 February 2009
- journal article
- editorial
- Published by Wiley in Angewandte Chemie International Edition
- Vol. 48 (10), 1729-1731
- https://doi.org/10.1002/anie.200805454
Abstract
The light-induced formation of a covalent bond between an alkene group and a tetrazole moiety has been used for the selective labeling of proteins in vitro and in live cells. This bioorthogonal ligation initiated by brief irradiation with UV light leads to a fluorescent pyrazoline adduct (see scheme).Keywords
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