Purification and characterization of virginiae butanolide C-binding protein, a possible pleiotropic signal-transducer in Streptomyces virginiae.
- 1 January 1990
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 43 (6), 692-706
- https://doi.org/10.7164/antibiotics.43.692
Abstract
Virginiae butanolide C (VB-C) is an autoregulator which triggers virginiamycin production in Streptomyces virginiae. A new binding assay with tritium-labeled VB-C analogue (2,3-cis-2-(1''-hydroxy-[6'',7''-3H]heptyl)-3-(hydroxymethyl)butanolide) was developed and a specific VB-C binding protein was purified to homogeneity from crude extracts of S. virginiae by ammonium sulfate fractionation. DEAE-Sephacel and Sephadex G-100 column chromatographies, hydrophobic HPLC on phenyl 5PW and native polyacrylamide gel electrophoresis. The VB-C binding protein showed an apparent Mr of 35,800 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Mr of 26,000 .apprx. 44,000 on native molecular sieve HPLC, indicating the monomeric nature of the binding protein. The binding protein efficiently bound to a VB affinity column and eluted specifically by VB-C, which confirmed the specific nature of the binding protein. The binding activity decreased by 40% in the presence of genomic DNA from S. virginiae, indicating interaction between the VB-C binding protein and the DNA.This publication has 14 references indexed in Scilit:
- Purification of a 31,000-dalton insulin-like growth factor binding protein from human amniotic fluid. Isolation of two forms with different biologic actions.Journal of Biological Chemistry, 1988
- Purification and partial characterization of a novel binding protein for retinoic acid from neonatal rat.Journal of Biological Chemistry, 1988
- Effect of B-factor and its analogues on rifamycin biosynthesis in Nocardia sp.The Journal of Antibiotics, 1988
- Juvenile‐hormone‐binding protein from the hemolymph of Galleria mellonella (L)European Journal of Biochemistry, 1987
- The structure of inducing factors for virginiamycin production in Streptomyces virginiae.The Journal of Antibiotics, 1987
- Affinity isolation of the interleukin-3 surface receptorBiochemical and Biophysical Research Communications, 1986
- Electrophoretic characterization of purified bovine, porcine, murine, rat, and human uterine estrogen receptors.Journal of Biological Chemistry, 1985
- B-factor, an essential regulatory substance inducing the production of rifamycin in a Nocardia sp.The Journal of Antibiotics, 1984
- Interspecific inducers of cytodifferentiation and anthracycline biosynthesis from Streptomyces bikinensis and S. cyaneofuscatusBiotechnology Letters, 1983
- Mutants blocked in streptomycin production in Streptomyces griseus - the role of A-factor.The Journal of Antibiotics, 1982