Transporting Renal Epithelium: Culture in Hormonally Defined Serum-Free Medium
- 21 November 1980
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 210 (4472), 912-914
- https://doi.org/10.1126/science.7434005
Abstract
A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.This publication has 17 references indexed in Scilit:
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