Whole genome amplification from a single cell: implications for genetic analysis.
- 1 July 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (13), 5847-5851
- https://doi.org/10.1073/pnas.89.13.5847
Abstract
We have developed an in vitro method for amplifying a large fraction of the DNA sequences present in a single haploid cell by repeated primer extensions using a mixture of 15-base random oligonucleotides. We studied 12 genetic loci and estimate that the probability of amplifying any sequence in the genome to a minimum of 30 copies is not less than 0.78 (95% confidence). Whole genome amplification beginning with a single cell, or other samples with very small amounts of DNA, has significant implications for multipoint mapping by sperm or oocyte typing and possibly for genetic disease diagnosis, forensics, and the analysis of ancient DNA samples.Keywords
This publication has 24 references indexed in Scilit:
- Sperm typing allows accurate measurement of the recombination fraction between D3S2 and D3S3 on the short arm of human chromosome 3Genomics, 1992
- A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations.1992
- Ordering three DNA polymorphisms on human chromosome 3 by sperm typingGenomics, 1991
- Using PCR in preimplantation genetic disease diagnosisHuman Reproduction, 1991
- PCR analysis of DNA sequences in single cells: Single sperm gene mapping and genetic disease diagnosisGenomics, 1990
- Multilocus ordering strategies based on sperm typingAnnals of Human Genetics, 1990
- Single-sperm typing: determination of genetic distance between the G gamma-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers.Proceedings of the National Academy of Sciences, 1989
- BIOPSY OF HUMAN PREIMPLANTATION EMBRYOS AND SEXING BY DNA AMPLIFICATIONThe Lancet, 1989
- Amplification and analysis of DNA sequences in single human sperm and diploid cellsNature, 1988
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985