LYSIS OF TUMOR-CELLS BY ANTIBODY AND COMPLEMENT .7. COMPLEMENT-DEPENDENT RB-86 RELEASE - NONLETHAL EVENT

Abstract

The guinea pig hepatoma (line-1) treated with [rabbit] anti-Forssman antibody (TA) and GPC [guinea pig complement] sequentially released 86Rb, 14C from 14C aminoisobutyric acid [AIB] and failed to exclude trypan blue. Incubation of TA with fluid phase GPC for 1 min caused maximal 86Rb release; if the GPC was removed at this time, the cells were not subsequently killed. Using a number of naturally occurring human sera deficient in a complement component, 86Rb release was shown to require the binding of the complement components 1-8 [C1-C8], but there was no absolute requirement for C9. Irreversible damage to the cell as measured by 14C AIB release or uptake of trypan blue required the complete sequence of complement components. 86Rb release is apparently not a reliable indicator of cytotoxicity.