Oxidative and Phosphorylative Activities of Mitochondria Isolated From Cotton Hypocotyls
Open Access
- 1 December 1968
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 43 (12), 1996-2000
- https://doi.org/10.1104/pp.43.12.1996
Abstract
Mitochondria isolated from 2 strains of cotton plant hypocotyls (Gossypium hirsutum L. var. Rex smooth leaf and Rex glandless) were examined for their oxidative phosphorylation activities. Bovine serum albumin at a relatively high concentration was essential in the extraction medium for the isolation of oxidatively active mitochondria from both strains of cotton. Phosphorylation was obtained only with Rex glandless cotton mitochondria. This activity was low in comparison to the mitochondria isolated from soybeans (Glycine max L. var. Lee). The endogenous gossypol content was found to be much higher in the Rex smooth leaf tissue than in the Rex glandless tissue. In turn, comparable gossypol differences were found associated with their respective mitochondrial fractions. Exogenous gossypol uncoupled succinate oxidation with active mitochondria isolated from soybeans. Gossypol as a possible uncoupler is discussed and compared to carbonyl cyanide, m-chlorophenyl hydrazone and 2,4-dinitrophenol.Keywords
This publication has 6 references indexed in Scilit:
- Properties of Higher Plant Mitochondria. I. Isolation and Some Characteristics of Tightly-coupled Mitochondria from Dark-grown Mung Bean HypocotylsPlant Physiology, 1967
- Oxidative and Phosphorylative Activities of Mitochondria Isolated from Pea Root TissuesPlant Physiology, 1965
- Isolation of metabolically-active subcellular particles from etiolated cotton seedling hypocotyls using bovine serum albumin in preparative mediumPlant Physiology, 1961
- Reactions of proteins with gossypolArchives of Biochemistry and Biophysics, 1959
- The Respiratory Chain and Oxidative PhosphorylationAdvances In Enzymology and Related Subjects Of Biochemistry, 1956
- The Estimation of Dehydrogenases in Plant Tissue.Plant Physiology, 1954